In the electrophoresis part of MBRC
different technique are used:
ü Polyacrylamide
Gel Electrophoresis (PAGE)
ü Sodium Dodecyl Sulfate - Polyacrylamide Gel
Electrophoresis (SDS-PAGE)
ü Two-dimensional Gel Electrophoresis (2-D
electrophoresis)
ü Western Blotting
Protein
electrophoresis is a method for analyzing
the proteins in a fluid or an extract
,the binding of SDS to the polypeptide chain imparts an even distribution of
charge per unit mass, thereby resulting in a fractionation by approximate size
during electrophoresis.Gel electrophoresis is often performed in combination with immunoblotting to
give additional information about a specific protein.
The western
blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology
disciplines to detect specific proteins in a sample of tissue homogenate or extract.
Two-dimensional gel
electrophoresis, abbreviated as 2-DE or 2-D
electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins.In the first dimension, molecules are separated linearly according
to their isoelectric point. In the second dimension, the molecules are then
separated according to molecular mass.The result of this is a gel with proteins
spread out on its surface. These proteins can then be detected by a variety of
means, but the most commonly used stains are silver and Coomassie Brilliant Blue.
In quantitative proteomics, Software ImageMaster analyze bio-markers by quantifying individual
proteins, and showing the separation between one or more protein
"spots" on a scanned image of a 2-DE gel. Additionally, these tools
match spots between gels of similar samples to show, for example, proteomic differences
between early and advanced stages of an illness
.