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  • Kermanshah Biliogy research Center
  • Kermanshah Biliogy research Center
  • Kermanshah Biliogy research Center

Protein Electrophoresis

 

In the electrophoresis part of MBRC different technique are used:

ü  Polyacrylamide Gel Electrophoresis (PAGE)

ü  Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE)

ü  Two-dimensional Gel Electrophoresis (2-D electrophoresis)

ü  Western Blotting

 

Protein electrophoresis is a method for analyzing the proteins in a fluid or an extract ,the binding of SDS to the polypeptide chain imparts an even distribution of charge per unit mass, thereby resulting in a fractionation by approximate size during electrophoresis.Gel electrophoresis is often performed in combination with immunoblotting to give additional information about a specific protein.

The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract.

Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins.In the first dimension, molecules are separated linearly according to their isoelectric point. In the second dimension, the molecules are then separated according to molecular mass.The result of this is a gel with proteins spread out on its surface. These proteins can then be detected by a variety of means, but the most commonly used stains are silver and Coomassie Brilliant Blue.

In quantitative proteomics, Software ImageMaster analyze bio-markers by quantifying individual proteins, and showing the separation between one or more protein "spots" on a scanned image of a 2-DE gel. Additionally, these tools match spots between gels of similar samples to show, for example, proteomic differences between early and advanced stages of an illness