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  • Kermanshah Biliogy research Center
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Farhad Oubari

 


 

 PERSONAL:

 

Name: Farhad Oubari

Title: Msc of Hematology (Researcher)

Date of Birth: 21/09/1978

Nationality: Iranian

Office address: Department of  Stem cell and Leukemia  ,Medical Biology Research Center & Department of Laboratory Sciences ,Paramedical Faculty , Kermanshah University of Medical Sceinces, Kermanshah , Iran.

E-mail:Farhadobari@yahoo.com & Foubari@mbrc.ac.ir

Tel: + 98 (83) 34243452

Fax: + 98 (83) 34210365

Cell Phone:+98  0918 130 7527

 

 EDUCATION:

        Degree                                                    Institute

1.Msc in hematology               Blood Transfusion Research Center, High Institute for 

       Tehran, Iran                           Research and Education in Transfusion Medicine.

 2.BSc in Laboratory               Department of Laboratory Sciences, Shiraz University     

  Sciences, Shiraz, Iran                 of Medical Sciences

 

 

 Employment:

 

1. Molecular Genetic &  Cell Culture (Medical Biology Research Center)

2. Research Center  of  Bone Marrow Transplantation , Flow cytometry  lab (Kermanshah   

    University of Medical Sciences), Kermanshah-Iran                   

3.Department of laboratory Sciences, Para medical Faculty, Kermanshah University 

    of Medical Sciences, Kermanshah- Iran

4.Department of Stem Cells &Blood Malignancies (Medical Biology Research Center)

5. Blood Transfusion Research Center(Stem Cell), High Institute for Research and Education in Transfusion Medicine,Tehran,Iran.

 

 

Relevent course:

             Title                                         Center                                                             Date

1.Hematology courses      Shiraz University of medical Sciences                           2003-2005                                                

                                                

2. Practical hematology    Shiraz University of medical Sciences                           2003- 2005

                                                

3. Flow cytometry            Iranian Blood Transfusion Organization                         2010 - 2013                                                               

 

 

4.Hemtology courses                 Iranian Blood Transfusion Organization                  2010-2013

 

5. Practical Hematology            Iranian Blood Transfusion Organization                  2010-2013                                                                          

 

6.Immunohematology course    Iranian Blood Transfusion Organization                  2010-2013                                                                                    

   

 

 

Awards & merits:

 

1.      Selected as the best employee in 2003-2004

 

 

 WORK EXPERIENCE:

1. Immunophenotyping of leukemia and immunodeficiency patients by  flowcytometry

2. DNA analysis of leukemia and solid tumors

3. Cord Blood  processing and banking

4 .Work in Medical Laboratories

5 . PCR

6. Real Time – PCR

7. Flowcytometry

8.Cell Culture

9.Western- blotting & Northern blotting

10. RFLP

11. ELISA

12. Mesenchymal stem cell Separation and expansion (Culture)

13. Hematopoietic stem cell Separation and expansion (Culture)

14. Co culture

15.MTT assay and viability test

16.Computer Skills

17. Skills in diagnosis of hematologic malignancies

18.Primer Design

19.Immunohistochymical staining such as (Perl’s Stain ,MPO, …)

 

 PUBLICATIONS:

1.Farhad Oubari,Naser Amirizadeh, Hemen Mohammadpour, Mozhdeh Nakhlestani, Mahin Nikougoftar Zarif.  The important role of Flt3-l in exvivo expansion of hematopoietic stem cells following co-culture with mesenchymal stem cells. Cell Journal, 2015, Vol 17,No 2.

2. Oubari F, Nikougoftar Zarif M, Amirizadeh N, Shaiegan M, Atarodi K, Nakhlestani M,Mirzamoradi M, Fadai R, Golzade Kh.  Isolation and expansion of Mesenchymal Stem Cells from placent, Sci J Iran Blood Transfus Organ 2013; 10(3): 222-230.

 

3.Mohammad Reza Nikbakht , Mahin Nikougoftar Zarif ,Farhad Oubari(Corresponder author) , Kamran Mansouri, Roghayeh Hosseinikia , Mahboobe Hosseinikia , Ahmad Tajeh miri . Mesenchymal Stem Cells Transplantation: Immunobiology, Therapeutic Applications and Challenges- Review Article. Scientific Journal of Kurdistan University of Medical Sciences Volume 20, Issue 3, 15 July 2015, Pages 113-139.

4. MR Nikbakht ,M Nikougoftar Zarif, R Hosseinikia, K Mansouri, F Oubari(Corresponder author),A a Moghaddam,M Hosseinikia,A Tajehmiri. Overview on Some of Gene Mutations and ChromosomalAberrations in Acute Myeloblastic Leukemias  with Normal and Abnormal Karyotypes(Accepted-Inpress). J Clin Res Paramed Sci;2017.

 

5.Kamran Mansouri,Farhad Oubari,Somayeh Shamllo, Parya Ahmadi,Mahboob Kashani. Coagulation and Angiogenesis in Cancer . J Clin Res Paramed Sci 2016; 5(1):142-154 .

 

6. Abbas Mohammadi , Ebrahim  Abbasi Oshaghi , Arash Noori Sorkhani , Farhad Oubari , Roghaye Hosseini Kia , Ali Rezaei. Effect of Opium on Lipid Profile and Expression of Liver X Receptor Alpha (LXRα) in Normolipidemic Mouse, Food and Nutrition Sciences , 2012,3,249-254.

7.Hossein Javad , Hosseinin- zijoud Seyed ,Oubari Farhad , Mahmoodi Mehdi , Abbasi Oshaghi Ebrahim , Rajabi Gilan Nader , Ghasemi Seyed Ramin and Hashemi Behroz. Hepatoprotective effects of hydroalcoholic  extract of Allium hirtifolium (Persian shallot) in diabetic rats,J Basic Clin Physical pharmacol 2012;23(2):83-87.

8. Abbas Mohammadi , Nejad Mohammadi , Nader Najafi , Kazem Hasanpoor , Ramezan Ali Taheri , Farhad oubari  and Ebrahim Abbasi Oshaghi . Influence of Flaxseed on Some Biochemical Factors , Antioxidant Activity and Expression ABCG5 and ABCG8 Genes in the Liver of Diabetic Rat, British Journal of  Medicine  & Medical Research , 4(18):3571-3580,2014.

9.Hemen Moradi – Sardareh , Nejad Mohammadi , Farhad oubari , Mohammad Reza Nikbakht , Roghaye Hosseini Kia , Gholamreza Farnoosh , Reza Yari, Fateme mirzaei and Kazem Hassanpour. Biomedical  factors , MDA levels and Antioxidant activity in Opium addicted Hamsters, International Reserch Journal of Biological Sciences ,ISSN 2278-3202,Vol .3(2), 21-24,February (2014).

10.Abbas Mohammadi , Gholamreza  Farnoosh , Roghaye Hosseini  Kia , Farhad oubari  , Kazem Hassanpour , Hemen Moradi- Sardareh , Nejad  Mohammadi , Ebrahim Abbasi Oshaghi and Reza yari.  The In Vivo Biomedical  and Oxidative Change by Garlic and Ezetimibe Combination in Hypercholesterolemic  Mice,International  Research Journal of Biological Sciences,2278-3202,Manuscript No:ISCA-IRJBS-2013-603.

11. E. Abbasi oshaghi,, N. Zynolebadi, A. Mohammadi, M. Moradi, A. Tootoonchi, H.

Ghasemi, S. Hosseini-zijoud, F. Oubari, R. Hosseini kia, H. Ghasemi1- Goodarzi. Oral opium reduced lipid profiles with change in expression of liver x receptor alpha (LXRα). Research in Pharmaceutical Sciences, 2012;7(5).

 

12. Mahin Nikogoftar Zarif, Farhad Oubari. Micro RNA Profiling of  Megakaryocytic  Differentiated   Cord  Blood  CD133+ Cells(Processing).

 

13. F.Oubari(Corresponder author), R , Hosseinikia , M.Hosseinikia. Immunomodulatory Effects and Clinical Applications of Mesenchymal Stem Cells-Review Article.(Future Medicine Meeting Abstracts)October 21-23 2015Vol.10 No.07s.

 

14. Ahmad Tajehmiri,Saeid Rahbar,Farhad Oubari.Probiotics for Control of Nosocomial Infections.Biomedical Pharmacology Journal,Vol.8No.1,2015.

 

15. Roghayeh Hosseinikia, Mohammad Reza Nikbakht, Ali Asghar Moghadam, Ahmad Tajehmiri, Mahboobe Hosseinikia, Farhad Oubari(Corresponder author), Mahin Nikougoftar Zarif, Yahya Pasdar, Kamran Mansouri. Molecular and Cellular Interactions of Allogenic and Autologus Mesenchymal Stem Cells with Innate and Acquired Immunity and Their Role in Regenerative Medicine. IJHOSCR 11(1) - ijhoscr.tums.ac.ir – January, 1, 2017.

 

16. Nikbakht MR, Lachiniyan Sh, Rahbar S, Oubari F, Rostami Z and Tajehmiri A. Aflatoxin M1 Contamination in Traditional Yoghurts Produced in Guilan Province, Iran. Asian Journal of Pharmaceutical Research and Health Care,2016 ; Vol 8(1), 1-3.

 

17. Hosseini Kia , Ghahramani L, salehi M, Oubari F, Hosseinikia M, Kaveh M.H. Effect of a Healthy Nutrition Education Program on Self-Efficacy in Dietary Behavior among Female Students of Guidance School in Shiraz, Iran: Application of PRECEDE Model. Vol. 7, No. 5, Winter 2016, Pages 499-509.

18. Hossinikia R , Nikougoftar Zarif  M, Pasdar Y, Haghnazari  L , Hossinikia M ,Oubari F(Corresponder author),. Biology , Labeling , Immunogenetic Isolation of Mesenchymal Stem Cells and Their role in Adult Stem Cells Niche(Submitted –Under Review  ).

19. Kardide B, Oubari F , Mansouri K. Autophagy and its role in Cancer and Angiogenesis(In Press).

20.Hosseinikia M, Abdollahzadeh H, Oubari F, Hosseinikia R, Shabanpour M, Nachvak MS. .Assessment of nutritional risk factor and SES indicator on childhood cancer(Submitted –Under Review ).

21. Hosseinikia M, Abdollahzadeh H, Oubari F, Hosseinikia R, Shabanpour M, Nachvak MS.Assessment of qualitative and quantitative pattern of kebab intake as a potential carcinogenic risk factor in Iranian adults(Submitted –Under Review ).

 

International Congresses (Oral & Poster Presentation):

1. F . Obari , M. Nikougoftar Zarif , N . Amirizade , K . Atarodi , M. Nakhlestani , H . Mohammadpour , R. Hosseini Kia , M . Notghi , N . Razavi. FLT3-L ONLY IN CO CULTURE MANNER  IS  AS  EFFECTIVE  AS  CYTOKINE  COCKTAILS  FOR  HEMATOPOIETIC STEM  CELL  EXPANSION, WORD  CORD  BLOOD  CONGRESS  AND  INNOVATIVE THERAPIES  FOR  SICKLE  CELL  DISEASE,  MONACO,  OCTOBER 24-27, 2013.

2.Farhad Oubari(Corresponding – Presenter (oral)), Mohammad reza Nikbakht , Roghaye Hosseni Kia , Hemn Mohammadpour , Mahin Nikogoftar Zarif , Dariush Pormand , Fatemeh Darabi , Shahryar Aazimi , Mahbob Kashani.Expansion of umbilical cord blood hematopoietic  stem cell in co culture with placental derived mesenchymal stem cell,6th International Iranian Congress Labo ratory  & Clinic.

3. Farhad Oubari ( Corresponding – Presenter (oral)), mahin Nikougoftar Zarif , Mohammad reza Nikbakht, Roghaye Hosseni Kia, Fatemeh Darabi , Dariush Pormand , Mahbob Kashani , Mahboobeh  hosseini Kia , Shahriyar Aazimi. Isolation , expansion ,  osteogenic  and adipogenic  differentiation Mesenchymal Stem cells – Derived from placenta,   6th International Iranian Congress Laboratory  & Clinic. 

4. Farhad Oubari ( Corresponding – Presenter( Poster)),Roghaye Hosseini kia , Ebrahim Abbasi Oshaghi , Sajad obari, Mahboobe Hosseini Kia , Farzan Vafaee , Almas jamshidi.  Effect of Opium on Lipid Profile and Expression of Liver X Receptor Alpha (LXRα) in Normolipidemic Mouse.5th International &10th National Congress on Quality Improvement in Clinical Laboratory, April 23-26,2012.

5. Farhad oubari (Corresponding- Presenter (Oral)) , , Dr.Mohammad Reza Nikbakht, Roghayeh Hosseinikia Mahboobe Hosseinikia,Kamran mansouri. Stem Cells Therapy and challenges ahead .Yasuj Blood Diseases International Congress2015.

6. Farhad Oubari(Corresponding-Presenter (Poster)) , Mohammad Reza Nikbakht , Mahin Nikougoftar Zarif ,Roghayeh Hosseinikia ,Mahboobe Hosseinikia ,Kamran Mansouri. In vitro Osteogenic and Adipogenic Differentiation of Placenta- Derived Mesenchymal Stem Cells (PD-MSCs). International Mashhadstemcell congress 2015.

7.Mohammadreza Nikbakht,Farhad Oubari,Ali asghar Mogghadam,Roghayeh Hosseinikia,Mahboobe Hosseinikia.Effects of Pharmaceutical Composition of All-Trans Retionoic Acid ,Valproic Acid and 5-Azacitidin in Patients with Acute Myeloid Leukemia Diagnosed in West of Iran.International Congress of  Physiology and Pharmacology(Kashan2015).

8. Farhad Oubari(Corresponding Presenter(Poster),  Mohammadreza Nikbakht, Roghayeh Hosseinikia, Mahboobe Hosseinikia. Immunomodulatory Effects and Clinical Applications of Mesenchymal Stem Cells- Review Article.International Congress of Regenerative medicine Congress(Germany,Liepzing ,2015).

9. Oubari F , Hosseinikia R , Hosseinikia M .Expansion of  Umblical  Hematopoietic  Stem Cell following Mesenchymal Stem Cells Co-culture. World Stem Cells  Congress (USA ,Florida - Orlando ,2017).(Invited  Speaker)

BOOK:

* Farhad Oubari, Roghaye Hosseni Kia:  Red Cell Membrane and Disorders   (Persian), Published in Publications research Center of Iranian Blood transfusion Organization.

Teaching Course:

1. Hematology  in Students of  BSc laboratory Science (Department  of  laboratory  Science, Islamic Azad University, Borujerd  Branch, Borujerd Iran).

2. Hematology & Blood Bankig  in Students of  BSc laboratory Science    (Department  of laboratory  Sciences, Para medical Faculty, Kermanshah University of Medical Sciences, Kermanshah, Iran)

Researches  in hands

1.  Evaluation of Multidrug Resistant Protein -1 genes expression rate in hematopoietic stem cells derived cord blood in from concurrent culture of hematopoietic stem cells and placenta derived mesenchymal stem cells in presence of FLT3-L

2. The effect of quercetin supplementation on Total Antioxidant Capacity, IL-6 and gene Expression of SREBP in nonalcoholic fatty liver disease:A randomized, double-blind, clinical trial.

3. Effects of L-carnitine supplementation on gene Expression of PPARα, CPT1 and ACOX and some of inflammatory factors in patients with coronary artery disease: A randomized clinical trial.

4. Comparison of  the umbilical cord and bone marrow CD34 + cells expansion  in co-culture with mesenchymal stem cells isolated from the placenta and bone marrow in the presence of FLT3-L and assessment of  the differentiation potency of osteocytic, adipocytic and adipocytic  mesenchymal stem cells isolated from placenta and bone marrow.

5.Evaluation of TSGA10 Gene  in Hela Cancerous Cells and Determination of Pattern of miRNA 200 and 214 in Angiogenesis.

6. Comparison of the Inhibitory Effect of  STMN1 using the RNAi on Expansion ,Viability and Migration in Human Cancerous Cells.

7.Role of TSGA10 in Autophagy of Cervical Cancer Cells.

8.Study of TSGA10 Gene Expression in Acute Myeloblastic Leukemia and Determination of Pattern of miRNA221/222 in AML Patients.

9.The Effect of Quercein Supplementation on Liver Enzymes,Inflammatory Indices, Lipid Profile , and Body Composition Accompanied by Colori Control in Non Alcohlic Fatty Liver Disease:A Randomised , Double Blind Pilot Study(Adviser) .

Language Score:

 Type                                          Score                                                       Date                                       MCHE(MSRT)                                       61                                         22/06/2012-22/06/2014                                              

Lecture background;

1. Giving a lecture in the fifth International &10th National Congress on Quality  Improvement in Clinical Laboratory, Tehran-Iran( April 23-26,2012).

2.Giving a lecture in the sixth International Iranian Congress Laboratory  & Clinic, Tehran- Iran(Accepted first article as a lecture)( 12-14 February 2014).

3.Giving a lecture in the sixth International Iranian Congress Laboratory  & Clinic, Tehran- Iran(Accepted second  article as a lecture)( 12-14 February 2014).

4. Lecturing in blood bank (ABO & Rh groups , Ab identification and  Screening Ab) in       Assembly hall in  Kermanshah University of Medical  Sciences ,Kermanshah – Iran(28 February 2009).

5. Lecturing in transmitted disease through water and food in Kermanshah University of Medical Sciences, Kermanshah- Iran (25 July 2007).

6.Giving a lecture in Apheresis and Hemovigilance in Kermanshah University of Medical Sciences , Kermanshah – Iran(9 June 2014).

7. Lecturing in  one – day workshop  in laboratory diagnosis of Tuberculosis,Kermanshah University of Medical Sciences, Kermanshah –Iran (9 July 2008).

8. Giving a lecture in International Congress of  Blood Diseases and Clinical Administration , Yasuj – Iran 2015(11-14 May).       .

Certificate  in  participating  in  internal  seminars  and Congress:

1. The Fifth International &10th National Congress on Quality Improvement in Clinical Laboratory, Tehran-Iran( April 23-26,2012).

2. The Second International &7th National Congress on Quality Improvement

    in Clinical Laboratory, Tehran-Iran( April 23-26,2009).   

3. The First International &6th National Congress on Quality Improvement in  

   Clinical Laboratory, Tehran-Iran(16 February 2008).      

4.Participating certificate in Hematology workshop, Tehran-Iran  (23-26 April 2009).

5. Participating certificate in Diagnosis of  Blood  Malignancy workshop, Tehran-Iran(16 February 2008).

6. Participating certificate in Peripheral Blood Smear workshop, Tehran-Iran(16 February 2008).

7. One – day workshop in the blood bank in Hamadan University of medical sciences , Hamadan-Iran(1 June 2009 ).

8.Certificate of perspective course of the new employees in Kermanshah-Iran(18 March 2007).

9.Certificate of participation in blood bank as a lecture in the system  of blood groups in Kermanshah University of Medical Sciences, Kermanshah –Iran(28 February 2009 ).

10.Certificate of participation in blood bank ( Screening Ab & Ab Identification), Kermanshah University of Medical Sciences, Kermanshah – Iran(28 February 2009).

11. Provience workshop in laboratory  diagnosis of  Transmitted disease  through food (Salmonella , Shigella, Staph and Escherichia ), Kermanshah University of Medical Sciences, Kermanshah- Iran(25 July 2007).

12. One – day conference in Hematology , Kermanshah University of Medical Sciences , Kermanshah-Iran(26 February 2007).

13. One – day conference in Experiments of urine Biochemistry, Kermanshah University of Medical Sciences, Kermanshah – Iran( 3 March 2007).

14. One – day conference in the approaches and techniques in Immunology, Kermanshah University of Medical Sciences, Kermanshah – Iran (13 January 2007).

15. One – day workshop  in  Blood Bank (Products of Cell and Plasma & Cross match , Quality Control  of  equipments  of  Blood transporter  ,...)Kermanshah – Iran (4 August 2008).

16. One – day workshop in laboratory diagnosis of Tuberculosis, Kermanshah University of Medical Sciences, Kermanshah –Iran (9 July 2008).  

17. One – day educational workshop in laboratory diagnosis of transmitted disease through Food , Tehran-Iran(12 February 2007).  

18. Certificate of participating in 6th International Congress in Lab and Clinic, Tehran- Iran (12-14 February 2014).       

Education Background :

1. BSc Average(14.99  from  20) & Graduated in 2005.

2. MSc Average (17.16 from 20) & Graduated in 2013.

3. Job experience in all medical lab wards and Research sectors (1999-2016).

4. Work as a head expert from 2007 to 2009.

5. Selected as a Pathology and blood Transfusion committee chairman & Responsible for quality control from 2007- 2009.

6.Selected as a referee of  research projects in Medical Biology Research Center,Kermansh University of Medical Sceinces.

7.Selecte as referee two Journal (Babol and Kermanshah):

A:Journal of Kermanshah University of Medical Sciences.

B: Journal of Babol University of Medical Sciences

Interests:

1. Hematopoietic and Mesenchymal  Cell  Biology

2. Umbilical Cord Blood Stem cell (Differentiation and Expansion)

3. Bone Marrow Transplantation

4. Cell Culture and Differentiation

5. Methods of Molecular in Diagnosis of non Hematogical ( such as Cancers) and   Hematological  Malignancies ( Such as  Leukemia , Lymphoma,…)    

6. Isolation &Expansion and Differentiation Hematopoietic Stem cell

7.Isolation &Expansion and Differentiation Mesenchymal Stem cells

8. Immunophenotyping of Leukemia and Solid Tumors

9.Epigenetic and Molecular Hematology

10. Immunohistochemistry of Malignancies

11. Hematopoesis & Coagulation and Blood Banking (Immunohematology)

12.Cancer and Leukemia Stem Cell

13. Inducible Progenitor Stem Cell (iPS)

14. MicroRNA

 

Referees :

1. Dr. Mahin Nikougoftar Zarif

*Assistant  professor , Blood Transfusion Research Center, High  Institute  for Research  and Education  in Transfusion Medicine, Tehran – Iran.

E- mail: Nikougoftar @ ibto.ir

2.Dr Kamran  Mansouri

*Assistant professor of  Molecular Medicine,Kermanshah University of Medical Sciences,Kermanshah-Iran.

E-mail:kmansouri@kums.ac.ir

 

3- Dr. Habibollah  Golafshan 

*Assistant professor of Hematology and blood Banking, Shiraz University of Medical Sciences, Shiraz- Iran.

E- mail: golafshanh@yahoo.com

 

4. Dr . Naser Amirizadeh

*Associated professor , Blood Transfusion Research Center , High Institute for Research and Education In Transfusion Medicine, Tehran-Iran.

E-Mail: n. amirizadeh @ ibto . ir